Use of Markers Ki- 67, CD3 and CD79a as Immunohistochemistry’s Technique for Classification of Canine Lymphoma - Case Report

Lymphomas, cancers originating in components lymphopoietic, are very common in dogs. The most used diagnostic methods for canine lymphoma are histopathology and immunohistochemistry. Immunohistochemistry is a technique employing labeled antibodies able to identify antigens ̈in situ ̈, which score in this type of neoplasm and the degree of cell proliferation. The most commonly used markers for canine lymphoma are B lymphocytes (CD79a), T (CD3) lymphocytes and cell proliferation marker Ki -67. This paper reports a case of a dog, the English Bulldog breed, which was diagnosed lymphoma, and by immunophenotyping showed positive to negative for B (CD79a) T lymphocytes (CD3) and lymphocytes, and demonstrated a high degree of malignancy based the pattern of cell proliferation by Ki -67. Histological classification type of cell pattern was seen centroblastic representing high-grade malignancy.